Clinical Trial Tissue Services (CTTS)
About the services
Clinical Trial Tissue Services is a shared resource of Yale Cancer Center & Yale Pathology Tissue Services.
Mission
The mission of Clinical Trial Tissue Services is to provide the Yale community and clients around the world with high quality formalin-fixed paraffin embedded material (FFPE) to promote and facilitate research. Tissue is often requested for inclusion in a clinical trial, or for the correlative science associated with clinical trials requested in IRB/HIC approved protocols with specific requirements. All our tissue is handled in strict compliance with HIPAA regulations, university research policies, Pathology Department diagnostic requirements, and hospital bylaws.
Available to Yale researchers & external researchers
Services & procedures
Services
It is the policy of our department to retain all tissue blocks, so to satisfy investigators with indispensable material for research or clinical trial, we offer the following:
- Paraffin-embedded tissue block cores ranging from 1 mm to 4 mm
- Paraffin-embedded tissue block cores that are re-embedded into a labeled tissue block
- Paraffin-embedded recut slides at thickness requested
- Paraffin-embedded recut slides that are H&E stained
Procedures
For each work request, a standard CTTS Work Request form must be completed by the requesting investigator. The completed form gives us your ordering and shipping information and charging instructions, as well as patient-requested tissue specifications. CTTS strives to complete all requests within a time-sensitive manner.
Please email all Clinical Trial Tissue Service requests to CTTS@yale.edu or fax to 203-737-8388.
Reference materials
CTTS Internal Process flowchart
Flowchart highlighting steps in the process of completing an internal (Yale) request for tissue service.
Receive CTTS tissue request → Verify patient information → Obtain diagnostic slides → Attain pathology review → Secure best representative block → Cut/core block → Complete/update lab req form → De-identify pathology report → Prepare shipping courier label → Ship material → Scan & save documents → Bill for services provided → Enter BSM OnCore → Resolve tissue queries
CTTS External Process flowchart
Flowchart highlighting steps in the process of completing an external (non-Yale) request for tissue service.
Receive CTTS tissue request → Verify patient consent form → Obtain diagnostic slides → Attain pathology review → Secure best representative block → Cut/core block → Prepare shipping courier label → Ship material → Scan & save documents → Bill for services provided
Contacts
Anita Huttner, MD, Medical Director
Katarzyna Kanarek, MS, Supervisor, CTTS Laboratory; CTTS Technical Director
Prital Patel, Clinical Technologist 2
Sarah Kubek, Research Assistant 2 MS
Location: LMP 100
Tel: 203-737-2378
Fax: 203-737-8388
Mailing:
Yale School of Medicine
Department of Pathology
PO Box 208023
New Haven, CT 06520-8023
To ship specimens:
Yale School of Medicine
Department of Pathology
310 Cedar St., LMP 100
New Haven, CT 06520
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Services
Cellular and mitochondrial respiration and bioenergetics
Cellular and mitochondrial respiration (OCR) and acidification (ECAR) are assessed with the Agilent Seahorse XF Pro 96. In a 96-well plate format, real-time measurements of cellular respiration, oxidative phosphorylation, mitochondrial leak, and mitochondrial transport are assessed as functional kinetic measurements of cellular bioenergetics. Changes in acidification are also observed simultaneously. An integrated Agilent BioTek Cytation5 imager correlates cell number via Hoechst nuclear stain to respiration for more accurate normalization of respiration.
Located within Chemical Metabolism CoreRebecca Cardone, MS
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Instrument/equipment
BD LSR Fortessa X20
The Fortessa analyzer has 4 lasers, including V 405 nm, B 488 nm, YG 561 nm, and R 640 nm. It can detect 16 colors.
Located within Science Hill Flow Cytometry FacilityFreya Wencker, PhD
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Instrument/equipment
BD Symphony A1 analyzer
Analyze from single tubes or 96-well plates with 45 lasers (violet, blue, yellow/green, red) and 64 detectors (6 violet, 2 blue, 4 yellow/green, 3 red).
Located within West Campus Flow Cytometry Facility