Purification

Every effort is made to maximize quality and yield in our unpurified oligos; they are suitable for most PCR and sequencing applications without further purification. When higher purity is required, we offer two types of purification.

Reverse-phase cartridge purification 

The trityl group at the 5' end of the molecule is left on at the completion of synthesis and is used as a hydrophobic "handle" to fish out the desired full-length chains. Failure sequences, lacking a trityl group, will then be removed and the oligo simultaneously desalted.

If you prefer to perform this type of purification yourself, we can provide a protocol for this procedure. In this case, YOU MUST SPECIFY THAT THE OLIGOS ARE TO BE RUN "TRITYL ON."

Reverse-phase HPLC purification 

Offered for DNA oligos <100 bases. High-performance liquid chromatography (HPLC) purification typically adds 3-5 business days to turnaround time. HPLC of modified DNA oligos may require an initial desalting/cartridge purification step before HPLC, so an additional charge may apply.