Core/facility:
Keck Oligo Synthesis Resource
Protocols
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- PAGE purification protocol (PDF)
- Desalting protocol using Glen-Pak cartridges
- Glen-Pak purification protocol for DMT-ON oligos
- Cleavage and deprotection of unmodified trityl-ON DNA oligos
- Cleavage and deprotection of halogenated trityl-ON DNA oligos
- Cleavage and deprotection of 3'ddC trityl-ON DNA oligos
- Manual removal of 5'-trityl (DMT) from trityl-ON DNA oligos following deprotection
- Removal of 2'-protecting groups (TOM and TBDMS) from RNA oligos