Core/facility: Yale Flow Cytometry Facility

FlowJo Site License for Yale

  1. Research Cores Directory
  2. Yale Flow Cytometry Facility
  3. FlowJo Site License for Yale

The Yale Flow Cytometry Facility administers a site license, and seats are billed monthly. As of 08/10/2026, requests for FlowJo can be made in PPMS. Please see below for steps to acquire a seat license.

Frequently asked questions

1. Is this free? No, this is not free. PI's will be charged monthly for all personal licenses and lab computers associated with their account. PI's will be charged regardless of when a computer is registered in that cycle. PI's have the option to disengage associated personnel at any point by contacting facility managers, but please note that changes to charges will be reflected in the subsequent month's billing. Please check with your PI to see if they are participating before requesting access to the FlowJo portal.

A license "seat" can take two forms:

  • A personal license seat that entitles them to use FlowJo on up to 4 devices of their choice.
  • A workstation license seat that is tied to that computer and allows anybody to use that workstation software so long as they have a yale.edu email address and have registered. Trainees who only wish to use a workstation can register a "free" account without obligating the lab to pay for their personal devices.

2. How can I sign up? 

The first step to acquiring a FlowJo seat license is completing a request form in PPMS. Once you log in to PPMS Flow Cytometry Facility click on the Request tab on the top navigation bar. Scroll down to the bottom for Project Creation Requests and click on the FlowJo Seat License Request. Please fill out the form. Note: the title of your project in the form should be FlowJo Account. Once your request has been approved the facility will provide and activate your account.

If you are an investigator who wishes to use multiple devices and have already confirmed that your lab is participating, then please first ensure that you have downloaded v10.5 or later. Earlier versions of FlowJo are not compatible with Portal access. 

  • Point your browser to the FlowJo page. It is necessary to use your Yale domain email address during your registration.
  • Open FlowJo software and choose "Preferences" icon shaped like a heart on the upper right, and then the "License" button again on the upper right. This instructive pdf may be helpful. If your computer has been used previously using the prior HWA system, you will need to remove that serial license number located in the middle right of that tab. Otherwise, simply click the "FlowJo Portal Sign In" button at the top and enter your personal Portal account credentials. The tab will then update to indicate that the device has been authorized.
  • To create a computer workstation within the lab for multiple users, PIs must contact core facility staff to enable this special feature. This page reviews how PIs can then manage that workstation within the lab account.

3. How can I switch the devices associated with my personal license seat? Once you have obtained a license seat (see above), then the devices you've used to access FlowJo through the portal will become associated with your license seat. The first four hardware addresses of the devices you sign in on will be added to your list of Authorized Devices. However, you can manage your devices by signing into cloud.flowjo.com and selecting the Manage Devices tab.