Core/facility: Electron Microscopy Facility at CCMI Offering: EM CCMI instruments

EM CCMI: Sample preparation

The following instruments are available on a first-come, first-served basis. Please contact facility staff to sign up.

  • FEI Vitrobot Mark IV
  • Leica ultramicrotomes
  • Leica EM HPM 100 high-pressure freezer/AFS2

Sample preparation

Aquilos 2 Cryo-FIB
The Chameleon from SPT Labtech
Leica EM HPM 100 high-pressure freezer
Leica EM AFS2 freeze substitution machine
Leica EM UC7 ultramicrotome
Vitrobot Mark IV
Bal-Tec SCD 005 sputter coater
Gatan Solarus Advanced Plasma System (model 950)
 

Aquilos 2 Cryo FIB apparatus

Aquilos 2 Cryo-FIB

Dedicated to the preparation of thin, electron-transparent lamellas for high-resolution cellular cryo electron tomography. 

Chameleon SPT Labtech apparatus

The Chameleon from SPT Labtech

The Chameleon facilitates cryo EM sample preparation at picoliter volumes using self-wicking grids. This method enables rapid freezing (a few hundred milliseconds) after deposition, thus potentially solving preferred-orientation and protein-aggregation problems. 

Leica high-pressure freezer

Leica EM HPM 100 high-pressure freezer

Leica freeze substitution machine

Leica EM AFS2 freeze substitution machine

Leica ultramicrotome

Leica EM UC7 ultramicrotome

Vitrobot apparatus

FEI Vitrobot Mark IV

Sputter coater apparatus

Bal-Tec SCD 005 sputter coater

Plasma system apparatus

Gatan Solarus Advanced Plasma System (model 950)