Publications
Explore publications from the core on peripheral artery disease; myocardial infarction; molecular and multi-modality imaging; adipose tissue; and diseases of the aorta, brain, lung, and liver.
Contents
Peripheral artery disease model (hindlimb ischemic model in mice)
MicroCT in myocardial infarction (coronary angiogram in rodents)
Molecular imaging & multi-modality imaging
MicroCT in aortic disease
MicroCT in brain disease (cerebral angiogram)
MicroCT in lung disease (pulmonary angiogram)
MicroCT in liver disease (portal angiogram)
Peripheral artery disease model (hindlimb ischemic model in mice)
Corti F, Wang Y, Rhodes JM, Atri D, Archer-Hartmann S, Zhang J, Zhuang ZW, Chen D, Wang T, Wang Z, Azadi P, Simons M. N-terminal syndecan-2 domain selectively enhances 6-O heparan sulfate chains sulfation and promotes VEGFA165-dependent neovascularization. Nat Commun. 2019 Apr 5;10(1):1562.
Ricard N, Zhang J, Zhuang ZW, Simons M. Isoform-Specific Roles of ERK1 and ERK2 in Arteriogenesis. Cells. 2019 Dec 21;9(1):38.
Boutagy NE, Ravera S, Papademetris X, Onofrey JA, Zhuang ZW, Wu J, Feher A, Stacy MR, French BA, Annex BH, Carrasco N, Sinusas AJ. Noninvasive In Vivo Quantification of Adeno-Associated Virus Serotype 9-Mediated Expression of the Sodium/Iodide Symporter Under Hindlimb Ischemia and Neuraminidase Desialylation in Skeletal Muscle Using Single-Photon Emission Computed Tomography/Computed Tomography. Circ Cardiovasc Imaging. 2019 Jul;12(7):e009063.
Zhou HJ, Xu Z, Wang Z, Zhang H, Zhuang ZW, Simons M, Min W. UMOylation of VEGFR2 regulates its intracellular trafficking and pathological angiogenesis. Nat Commun. 2018 Aug 17;9(1):3303
Budatha M, Zhang J, Zhuang ZW, Yun S, Dahlman JE, Anderson DG, Schwartz MA. Inhibiting Integrin α5 Cytoplasmic Domain Signaling Reduces Atherosclerosis and Promotes Arteriogenesis. J Am Heart Assoc. 2018 Jan 30;7(3):e007501.
Landskroner-Eiger S, Qiu C, Perrotta P, Siragusa M, Lee MY, Ulrich V, Luciano AK, Zhuang ZW, Corti F, Simons M, Montgomery RL, Wu D, Yu J, Sessa WC. Endothelial miR-17∼92 cluster negatively regulates arteriogenesis via miRNA-19 repression of WNT signaling. Proc Natl Acad Sci U S A. 2015 Oct 13;112(41):12812-7.
Morrison AR, Yarovinsky TO, Young BD, Moraes F, Ross TD, Ceneri N, Zhang J, Zhuang ZW, Sinusas AJ, Pardi R, Schwartz MA, Simons M, Bender JR. Chemokine-coupled β2 integrin-induced macrophage Rac2-Myosin IIA interaction regulates VEGF-A mRNA stability and arteriogenesis. J Exp Med. 2014 Sep 22;211(10):1957-68.
Lanahan AA, Lech D, Dubrac A, Zhang J, Zhuang ZW, Eichmann A, Simons M. PTP1b is a physiologic regulator of vascular endothelial growth factor signaling in endothelial cells. Circulation. 2014 Sep 9;130(11):902-9.
Stacy MR, Yu DY, Maxfield MW, Jaba IM, Jozwik BP, Zhuang ZW, Lin BA, Hawley CL, Caracciolo CM, Pal P, Tirziu D, Sampath S, Sinusas AJ. Multimodality imaging approach for serial assessment of regional changes in lower extremity arteriogenesis and tissue perfusion in a porcine model of peripheral arterial disease. Circ Cardiovasc Imaging. 2014 Jan;7(1):92-9.
Cristofaro B, Shi Y, Faria M, Suchting S, Leroyer AS, Trindade A, Duarte A, Zovein AC, Iruela-Arispe ML, Nih LR, Kubis N, Henrion D, Loufrani L, Todiras M, Schleifenbaum J, Gollasch M, Zhuang ZW, Simons M, Eichmann A, le Noble F. Dll4-Notch signaling determines the formation of native arterial collateral networks and arterial function in mouse ischemia models. Development. 2013 Apr;140(8):1720-9.
Moraes F, Paye J, Mac Gabhann F, Zhuang ZW, Zhang J, Lanahan AA, Simons M. Endothelial cell-dependent regulation of arteriogenesis. Circ Res. 2013 Oct 12;113(9):1076-86.
Deng Y, Larrivée B, Zhuang ZW, Atri D, Moraes F, Prahst C, Eichmann A, Simons M. Endothelial RAF1/ERK activation regulates arterial morphogenesis. Blood. 2013 May 9;121(19):3988-96, S1-9.
Tirziu D, Jaba IM, Yu P, Larrivée B, Coon BG, Cristofaro B, Zhuang ZW, Lanahan AA, Schwartz MA, Eichmann A, Simons M. Endothelial nuclear factor-κB-dependent regulation of arteriogenesis and branching. Circulation. 2012 Nov 27;126(22):2589-600.
Hedhli N, Dobrucki LW, Kalinowski A, Zhuang ZW, Wu X, Russell RR 3rd, Sinusas AJ, Russell KS. Endothelial-derived neuregulin is an important mediator of ischaemia-induced angiogenesis and arteriogenesis.Cardiovasc Res. 2012 Mar 1;93(3):516-24.
Zhuang ZW, Shi J, Rhodes JM, Tsapakos MJ, Simons M. Challenging the surgical rodent hindlimb ischemia model with the miniinterventional technique. J Vasc Interv Radiol. 2011 Oct;22(10):1437-46.
Takeda Y, Costa S, Delamarre E, Roncal C, Leite de Oliveira R, Squadrito ML, Finisguerra V, Deschoemaeker S, Bruyère F, Wenes M, Hamm A, Serneels J, Magat J, Bhattacharyya T, Anisimov A, Jordan BF, Alitalo K, Maxwell P, Gallez B, Zhuang ZW, Saito Y, Simons M, De Palma M, Mazzone M. Macrophage skewing by Phd2 haplodeficiency prevents ischaemia by inducing arteriogenesis. Nature. 2011 Oct 9;479(7371):122-6.
Murakami M, Nguyen LT, Hatanaka K, Schachterle W, Chen PY, Zhuang ZW, Black BL, Simons M. FGF-dependent regulation of VEGF receptor 2 expression in mice. J Clin Invest. 2011 Jul;121(7):2668-78.
MicroCT in myocardial infarction (coronary angiogram in rodents)
Wang Y, Wu B, Lu P, Zhang D, Wu B, Varshney S, Del Monte-Nieto G, Zhuang Z, Charafeddine R, Kramer AH, Sibinga NE, Frangogiannis NG, Kitsis RN, Adams RH, Alitalo K, Sharp DJ, Harvey RP, Stanley P, Zhou B. Uncontrolled angiogenic precursor expansion causes coronary artery anomalies in mice lacking Pofut1. Nat Commun. 2017 Sep 18;8(1):578.
Zhuang ZW, Huang Y, Ju R, Maxfield MW, Ren Y, Wang X, Wang X, Stacy MR, Hwa J. Molecular Imaging of Factor XIII Activity for the Early Detection of Mouse Coronary Microvascular Disease. Theranostics. 2019 Feb 20;9(5):1474-1489.
Bartulos O, Zhuang ZW, Huang Y, Mikush N, Suh C, Bregasi A, Wang L, Chang W, Krause DS, Young LH, Pober JS, Qyang Y. ISL1 cardiovascular progenitor cells for cardiac repair after myocardial infarction. Insight. 2016 Jul 7;1(10):e80920.
Kivelä R, Bry M, Robciuc MR, Räsänen M, Taavitsainen M, Silvola JM, Saraste A, Hulmi JJ, Anisimov A, Mäyränpää MI, Lindeman JH, Eklund L, Hellberg S, Hlushchuk R, Zhuang ZW, Simons M, Djonov V, Knuuti J, Mervaala E, Alitalo K. VEGF-B-induced vascular growth leads to metabolic reprogramming and ischemia resistance in the heart. EMBO Mol Med. 2014 Mar;6(3):307-21.
Jaba IM, Zhuang ZW, Li N, Jiang Y, Martin KA, Sinusas AJ, Papademetris X, Simons M, Sessa WC, Young LH, Tirziu D. NO triggers RGS4 degradation to coordinate angiogenesis and cardiomyocyte growth. J Clin Invest. 2013 Apr;123(4):1718-31.
Landskroner-Eiger S, Qiu C, Perrotta P, Siragusa M, Lee MY, Ulrich V, Luciano AK, Zhuang ZW, Corti F, Simons M, Montgomery RL, Wu D, Yu J, Sessa WC. Endothelial miR-17∼92 cluster negatively regulates arteriogenesis via miRNA-19 repression of WNT signaling. Proc Natl Acad Sci U S A. 2015 Oct 13;112(41):12812-7.
Tirziu D, Chorianopoulos E, Moodie KL, Palac RT, Zhuang ZW, Tjwa M, Roncal C, Eriksson U, Fu Q, Elfenbein A, Hall AE, Carmeliet P, Moons L, Simons M. Myocardial hypertrophy in the absence of external stimuli is induced by angiogenesis in mice. J Clin Invest. 2007 Nov;117(11):3188-97.
Chittenden TW, Claes F, Lanahan AA, Autiero M, Palac RT, Tkachenko EV, Elfenbein A, Ruiz de Almodovar C, Dedkov E, Tomanek R, Li W, Westmore M, Singh JP, Horowitz A, Mulligan-Kehoe MJ, Moodie KL, Zhuang ZW, Carmeliet P, Simons M. Selective regulation of arterial branching morphogenesis by synectin. Dev Cell. 2006 Jun;10(6):783-95.
Molecular imaging & multi-modality imaging
Boutagy NE, Ravera S, Papademetris X, Onofrey JA, Zhuang ZW, Wu J, Feher A, Stacy MR, French BA, Annex BH, Carrasco N, Sinusas AJ. Noninvasive In Vivo Quantification of Adeno-Associated Virus Serotype 9-Mediated Expression of the Sodium/Iodide Symporter Under Hindlimb Ischemia and Neuraminidase Desialylation in Skeletal Muscle Using Single-Photon Emission Computed Tomography/Computed Tomography. Circ Cardiovasc Imaging. 2019 Jul;12(7):e009063.
Mohy-Ud-Din H, Boutagy NE, Stendahl JC, Zhuang ZW, Sinusas AJ, Liu C. Quantification of intramyocardial blood volume with 99mTc-RBC SPECT-CT imaging: A preclinical study. J Nucl Cardiol. 2018 Dec;25(6):2096-2111.
Zhuang ZW, Huang Y, Ju R, Maxfield MW, Ren Y, Wang X, Wang X, Stacy MR, Hwa J. Molecular Imaging of Factor XIII Activity for the Early Detection of Mouse Coronary Microvascular Disease. Theranostics. 2019 Feb 20;9(5):1474-1489.
Urao N, Okonkwo UA, Fang MM, Zhuang ZW, Koh TJ, DiPietro LA. MicroCT angiography detects vascular formation and regression in skin wound healing. Microvasc Res. 2016 Jul;106:57-66.
Zhen W Zhuang ZW, Huang Y, Yang X, Martin KA, Hwa J, Simons M. CT Molecular Imaging of Perivascular Adipose Tissue and Its Linkage to Vascular Disease. Circulation. 2016;134:A19281
Criscione JM, Dobrucki LW, Zhuang ZW, Papademetris X, Simons M, Sinusas AJ, Fahmy TM. Development and application of a multimodal contrast agent for SPECT/CT hybrid imaging. Bioconjug Chem. 2011 Sep 21;22(9):1784-92.
Stacy MR, Yu DY, Maxfield MW, Jaba IM, Jozwik BP, Zhuang ZW, Lin BA, Hawley CL, Caracciolo CM, Pal P, Tirziu D, Sampath S, Sinusas AJ. Multimodality imaging approach for serial assessment of regional changes in lower extremity arteriogenesis and tissue perfusion in a porcine model of peripheral arterial disease. Circ Cardiovasc Imaging. 2014 Jan;7(1):92-9.
Jiang Y, Zhuang ZW, Sinusas AJ, Staib LH, Papademetris X. Vessel connectivity using Murray's hypothesis. Med Image Comput Comput Assist Interv. 2011;14(Pt 3):528-36.
Zagorchev L, Oses P, Zhuang ZW, Moodie K, Mulligan-Kehoe MJ, Simons M, Couffinhal T. Micro computed tomography for vascular exploration. J Angiogenes Res. 2010 Mar 5;2:7.
Zhuang ZW, Gao L, Murakami M, Pearlman JD, Sackett TJ, Simons M, de Muinck ED. Arteriogenesis: noninvasive quantification with multi-detector row CT angiography and three-dimensional volume rendering in rodents. Radiology. 2006 Sep;240(3):698-707.
MicroCT in aortic disease
Chen PY, Qin L, Li G, Malagon-Lopez J, Wang Z, Bergaya S, Gujja S, Caulk AW, Murtada SI, Zhang X, Zhuang ZW, Rao DA, Wang G, Tobiasova Z, Jiang B, Montgomery RR, Sun L, Sun H, Fisher EA, Gulcher JR, Fernandez-Hernando C, Humphrey JD, Tellides G, Chittenden TW, Simons M. Smooth Muscle Cell Reprogramming in Aortic Aneurysms. Cell Stem Cell. 2020 Apr 2;26(4):542-557.e11.
Cuomo F, Ferruzzi J, Agarwal P, Li C, Zhuang ZW, Humphrey JD, Figueroa CA. Sex-dependent differences in central artery haemodynamics in normal and fibulin-5 deficient mice: implications for ageing. Proc Math Phys Eng Sci. 2019 Jan;475(2221):20180076.
MicroCT in brain disease (cerebral angiogram)
Vrselja Z, Daniele SG, Silbereis J, Talpo F, Morozov YM, Sousa AMM, Tanaka BS, Skarica M, Pletikos M, Kaur N, Zhuang ZW, Liu Z, Alkawadri R, Sinusas AJ, Latham SR, Waxman SG, Sestan N. Restoration of brain circulation and cellular functions hours post-mortem. Nature. 2019 Apr;568(7752):336-343.
MicroCT in lung disease (pulmonary angiogram)
Ramachandra AB, Sharma P, De Man R, Nikola F, Guerrera N, Doddaballapur P, Cavinato C, Choi R, Raredon MSB, Szafron JM, Zhuang ZW, Barnthaler T, Justet A, Akingbesote ND, Abu Hussein NS, Diggs L, Perry RJ, Adams TS, Singh I, Kaminski N, Yan X, Tellides G, Humphrey JD, Manning EP. Hypoxia-Induced Cardiopulmonary Remodeling and Recovery: Critical Roles of the Proximal Pulmonary Artery, Macrophages, and Exercise. bioRxiv [Preprint]. 2025 Feb 20:2025.02.15.638455. doi: 10.1101/2025.02.15.638455.
Syed M, Das P, Pawar A, Aghai ZH, Kaskinen A, Zhuang ZW, Ambalavanan N, Pryhuber G, Andersson S, Bhandari V. Hyperoxia causes miR-34a-mediated injury via angiopoietin-1 in neonatal lungs. Nat Commun. 2017 Oct 27;8(1):1173.
Petersen TH, Calle EA, Zhao L, Lee EJ, Gui L, Raredon MB, Gavrilov K, Yi T, Zhuang ZW, Breuer C, Herzog E, Niklason LE. Tissue-engineered lungs for in vivo implantation. Science. 2010 Jul 30;329(5991):538-41.
MicroCT in liver disease (portal angiogram)
Ma R, Gong L, Dong C, Utsumi T, Qi J, Zhuang ZW, Zhang X, Yang Y, McConnell MJ, Huang HC, Iwakiri Y. Hepatic Arterial Flow-induced Portal Tract Fibrosis in Portal Hypertension: The Role of VCAM-1 and Osteopontin-expressing Macrophages. Cell Mol Gastroenterol Hepatol. 2026 Jan 19:101728. doi: 10.1016/j.jcmgh.2026.101728.