BD Symphony C-5L
The core has a 5-laser Symphony located in TAC S533. Please see the configuration below for details and view the Symphony resource page. Another way of viewing the configuration is using Fluorofinder website, a useful panel building tool listing reagents from multiple companies. You can go to fluorofinder.com and follow Tools--> Flow Cytometry Panel Builder-->> Select your Instrument (after choosing Yale and School of Medicine FACS Facility from the drop-down menu).
The Symphony is equipped with a 561 nm yellow-green laser; this is different from the LSRII and Fortessa, which have 532 nm green lasers. You should expect your PE and PE-tandem dyes to appear brighter than they did before. Fewer antibodies might be required to avoid extreme adjustments on the sensitive detectors of this model.
Some fluorescent proteins that were barely detectable with a 532 nm laser will appear significantly brighter with 561 nm excitation. mCherry, mKate, and mRaspberry will perform much better with this laser line.
The Facility offers Symphony training for experienced LSRII/Fortessa users. Whether you've trained on the instrument before the pandemic and are looking for a refresher, or you've just decided you want to start using our sensitive 5 to 6-laser cytometers, our training video will raise your confidence to use the Symphony. Please contact Jennifer Kelly for information about Symphony training and make a request in PPMS.
Symphony A, B, & C are available 24/7 once you are an approved, trained user.