High-throughput assay screening

Primary screening

Primary screening typically generates single point data using one concentration for each compound or siRNA. We normalize screening data relative to the control populations on each plate, and provide plate-based summary statistics for an evaluation of screen performance. Hit selection strategies to fit your project goals are implemented. Investigators are responsible for procuring reagents to perform their screen whereas the Center is responsible for standard laboratory consumables (pipet tips, assay plates). The price for running and analyzing a primary screen typically reflects a combination of a fee per assay plate plus an hourly fee when Center staff are needed.  For Yale faculty, these fees are largely subsidized by the Center.

For a full description of our screening collections, visit our Small Molecule Collections or siRNA Library page.

Hit-picking and dose-response

Hit-picking is performed after a primary screen to retest active compounds and to rule out compounds which exhibit assay interference or toxicity, or to validate single siRNA duplexes for their effects. Dose-response studies, using serial dilutions of each compound, can be performed within a plate, or across a series of plates in cases where many compounds are being tested. Hit selection to fit your project goals are implemented. The price for setting up, running, and analyzing the results is a combination of a fee per assay plate and a Center hourly use fee, which are subsidized by the Center.