Self-service lab
Yulia Surovtseva
M–F, 8:30 AM–5:00 PM
About the service
YCMD’s self-service lab provides access to screening equipment as a lower-cost option to advance assay development and screening.
The self-service lab can be used to develop and optimize high-throughput assays, to assemble assays during screening, or to simply survey many assay conditions on a 384-well plate at once. Please note that while the equipment in the self-service lab is not sufficient for the entire screening, it can be used for part of screening activities to defray screening costs. On-call staff members will be available each day if additional support is needed.
The labs are available Monday through Friday, 8:30 AM to 5:00 PM.
Available to Yale researchers & external researchers
Reserve lab time
ReservationsTecan plate reader
How to use itResources
Instrumentation available in the self-service lab includes a complete set of repeater multichannel pipets (dispensing range 1 ul – 1 ml), multidrop liquid dispensers (dispensing range 1 ul – 390 ul), a plate centrifuge, plate mixers, and a multimode plate reader (luminescence, absorbance, fluorescence).
Also available is a tissue culture room equipped with two biosafety hoods, cell culture incubators, multichannel pipets, a multidrop liquid dispenser (dispensing range 2 ul – 390 ul), and a plate centrifuge.
Room temperature, 4C, -20C, and -80C storage space is available for short-term storage of reagents and supplies.
For now, office space is also available for use during a scheduled laboratory session.
The self-service lab does not include systems for compound transfer or an imaging system.
Consumables
YCMD will provide tips, pipets, Eppendorf and Falcon tubes, and reservoirs. PPE (lab coats, safety glasses, disposable gloves) is also provided by YCMD. You will be responsible for supplying all reagents required for your assay as well as all cell culture media and supplements. 384-well plates required for your assay can be purchased from the on-call staff member at cost.
Training & services
Training
Prior to access, you will have a 1-hour training session related to the work you will be doing, charged at the Yale-subsidized YCMD use rate. We ask that you do not train others on the instruments; all users must be trained by YCMD staff members before they are granted access to the self-service lab. To defray training expenses, multiple members from the same laboratory can be trained simultaneously.
Account request & reservations
- Please note that an active Workday COA (Chart of Accounts) number is required to set up an account.
- Ensure your device is on either Yale local network or a Yale VPN connection.
- Open the Yale FOM (Facility Online Management) page.
- Log in with your NetID and password.
- Follow instructions to set up your FOM account.
- Please upload your COA to your FOM account. Please note this is not automated payment authorization. However, FOM calendar records will be used for YCMD monthly invoicing, so please make sure that reservation reflects the actual use time. If you need to cancel your time, please be sure to do so before the actual reservation time. A reservation can only be extended if no one has reserved it after your time.
- An updated biosafety form is required for each new COA.
- On your “User Home” page, please expand Yale Center for Molecular Discovery to find the two pages: Self-service Biochemistry Lab and Self-service Cell Culture lab.
- Please select the Lab and place your reservation. Reach out to us if you have any issue along the way. Thanks!
Rates
Self-service lab use fee: $50 per hour for Yale users, $120 per hour for external users
Full-service YCMD use fee: $130 per hour for Yale users, $350 per hour for external users
Empty 384-well or 96-well plates: At cost, plate-dependent