Necropsy Service
About the service
For research animals only
The necropsy service is a complete necropsy and tissue collection service available to internal and external customers with all required approvals in place. The service can be performed only on the following species: mice, rats, zebrafish and amphibians.
Other services include:
- Tissue decalcification and trimming for histology.
- Verification of tissue decalcification.
- Research necropsy and tissue harvest of other YALE research animal species post euthanasia by veterinary clinical services.
Available to Yale researchers & external researchers
Order form
Internal users, please go to LSOG 109 and fill out the in-person triplicate submission form, or email us. External users, please email us. Effective 12/1/25, All submissions will be made through the PPMS system.
Fixation protocols
Immersion & perfusion fixationGetting started
Contact us to discuss your necropsy needs before starting a project or submitting samples. Research necropsies are performed during normal business hours, unless extenuating circumstances have occurred. A valid COA or other charging instructions must be on file before your samples can be accepted.
Clinical pathology: Additional tests can be requested at the time of necropsy. For virology or PCR diagnostic testing, contact Sue Compton in the Department of Comparative Medicine Molecular and Serological Diagnostics Laboratory. Serum chemistry, complete blood count and urinalysis or bacteriology not offered through our Microbiology service are available through an outside company, Antech Diagnostics (1-800-872-1001). These samples may be submitted directly by the investigator or submitted through our service and billed to the investigator.
A valid COA or other charging instructions must be on file before your samples can be accepted.
For external customers, gross necropsy and histopathologic evaluation or clinical pathology interpretation, please contact Carmen Booth.
Rates
Mouse Research Pathology Support is the only USP at the Yale School of Medicine providing pathologic phenotyping by comparative veterinary pathologists. In addition to gross and histologic evaluation of animal models, we also provide a variety of high-quality histology services tailored to individual projects.
Rates effective 1/1/25.
| Services | Unit | Yale research investigator | External research investigator |
|---|---|---|---|
| C&T technical time | per hour | $79.50 | $119.72 |
| M&P technical time | per hour | $79.00 | $115.00 |
| Pathologist | per hour | $140.00 | $204.50 |
| Necropsy: Research: Rodent (supplies only), for research animals only | per necropsy | $5.00 | $116.00 |
| Histology: Tissue processing only | per sample | $10.00 | $0 |
| Histology: Tissue processing and embedding | per sample | $12.25 | $35.00 |
| Histology: Tissue processing, embedding, sectioning, 1st H&E Slide | per sample | $21.00 | $65.50 |
| Histology: Cut unstained section from block (1-2 sections on one slide) | per slide | $3.50 | $14.75 |
| Histology: H&E slide | per slide | $5.25 | $69.00 |
| Histology: Special stains, non-silver stain only; no sectioning | per slide | $11.75 | $45.00 |
| Histology: Silver stain only; no sectioning | per slide | $21.00 | $83.00 |
| Histology: Frozen sectioning | per section | $21.00 | $400.00 |
| Microbiology: Aerobic culture, including Pseudomonas screening culture, urine cultures, cecal screening culture | per sample | $88.00 | $0 |
| Microbiology: Anaerobic culture | per sample | $130.00 | $0 |
| Microbiology: Antibiotic sensitivity -- group 1 | per sample | $84.00 | $0 |
| Microbiology: Antibiotic sensitivity -- group 2 | per sample | $84.00 | $0 |
| Microbiology: Blood culture | per sample | $145.00 | $0 |
| Microbiology: Diff Quik stain | per sample | $34.00 | $0 |
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Services
Cellular and mitochondrial respiration and bioenergetics
Cellular and mitochondrial respiration (OCR) and acidification (ECAR) are assessed with the Agilent Seahorse XF Pro 96. In a 96-well plate format, real-time measurements of cellular respiration, oxidative phosphorylation, mitochondrial leak, and mitochondrial transport are assessed as functional kinetic measurements of cellular bioenergetics. Changes in acidification are also observed simultaneously. An integrated Agilent BioTek Cytation5 imager correlates cell number via Hoechst nuclear stain to respiration for more accurate normalization of respiration.
Located within Chemical Metabolism CoreRebecca Cardone, MS
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Instrument/equipment
Invitrogen Attune NXT Cell Analyzer
Analyze cells from tubes and plates with 4 lasers (violet, blue, yellow/green, red) and 14 detectors (6 violet, 2 blue, 3 yellow, 3 red).
Located within West Campus Flow Cytometry Facility -
Instrument/equipment
BD Symphony S6 Cell Sorter
Sort into 5 mL round tubes, 15 mL conical tubes, or plates (24-, 48-, and 96-well) with 5 lasers (UV, violet, blue, yellow/green, red) and 23 detectors (6 UV, 6 violet, 4 blue, 4 yellow/green, 3 red).
Located within West Campus Flow Cytometry Facility