BD Symphony S6 Cell Sorter
Available to Yale researchers & external researchers
Specifications
| Symphony S6 Fluorophore Specifications | ||||
| Fluorophore | Filter | |||
| UV - 355 nm | ||||
| BUV805 | 820/60 | 770 LP | ||
| BUV737 | 740/35 | 690 LP | ||
| BUV661 | 670/30 | 635 LP | ||
| BUV563 | 586/15 | 550 LP | ||
| BUV496 | 515/30 | 450 LP | QDot 525 | |
| BUV 396 | 379/28 | Spark UV 387 | ||
| Violet - 405 nm | ||||
| BV786 | 780/60 | 750 LP | Super Bright 780 | |
| BV711 | 710/50 | 690 LP | QDot 705, Super Bright 702 | |
| BV650 | 670/30 | 635 LP | Super Bright 645 | |
| BV605 | 610/20 | 600 LP | Super Bright 600, QDot 605, PacOrange | |
| BV480 | 470/14 | 470 LP | AF430, Horizon V500, VioGreen, BV510, Pac Blue | |
| BV421 | 431/28 | 410 LP | DAPI, Hoechst, Horizon 450, Alexa Fluor 405, CellTrace Violet, CFP, Zombie Violet, VioBlue, PacGreen, Sytox Blue, eFluor 450, Super Bright 436 | |
| Blue - 488 nm | ||||
| BB790 | 780/60 | 770 LP | PerCP Fire 806 | |
| BB700 | 750/30 | 690 LP | PerCp-Cy5-5 | |
| BB700 | 750/30 | 690 LP | PerCP | |
| BB700 | 750/30 | 690 LP | Vio700 | |
| BB630 | 610/20 | 600 LP | PE-eFluor 610 | |
| BB630 | 610/20 | 600 LP | NovaFluor Blue610/30S | |
| FITC | 515/20 | 505 LP | BB515, AF 488, GFP, CFSE, Yo Pro1, Calcein, VioBright 515, Spark Blue 574, eFluor 520 | |
| Yellow -Green - 561 nm | ||||
| PE-Cy7 | 780/60 | 750 LP | Pe Fire 810 | |
| PE-Cy5 | 670/30 | 635 LP | 7AAD, mPlum, Pe Cy5.5, PE Fire 640, PE Fire 700, | |
| PE-CF594 | 610/20 | 600 LP | mCherry(weak), mStrawberry, PE TxRed, PI, Alexa Fluor 594, PE-Vio615, ECD, PE/Dazzle 594, PE eFluor610, Nova Fluor Yellow 610 | |
| PE | 586/15 | 570 LP | PI, TRITC, DsRed, SYTOXOrange, Cy3, RFP, HRP, Calcein, Red Orange, mTangerine, Rhodamine, tdTomato, AF 555, Spark YG570, Spark YG 581, Spark YG 593, eFluor 570 | |
| Red - 633 nm | ||||
| APC-Cy7 | 780/60 | 750 LP | RPE Cy7, Cy7, APC-AF750, APC CF780, APC/FIRE750, APC FIRE 810, Spark NIR 718 | |
| APC-R700 | 730/45 | 690/LP | AF700, APC-AF700, APC Cy5.5, AF 680, AF 660, Spark NIR 685 | |
| APC | 670/30 | AF 647, Cy5, AF633, DRAQ5, R PE Cy5, eFluor 660, ToPro3, SPARK NIR 685 | ||
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Services
Cellular and mitochondrial respiration and bioenergetics
Cellular and mitochondrial respiration (OCR) and acidification (ECAR) are assessed with the Agilent Seahorse XF Pro 96. In a 96-well plate format, real-time measurements of cellular respiration, oxidative phosphorylation, mitochondrial leak, and mitochondrial transport are assessed as functional kinetic measurements of cellular bioenergetics. Changes in acidification are also observed simultaneously. An integrated Agilent BioTek Cytation5 imager correlates cell number via Hoechst nuclear stain to respiration for more accurate normalization of respiration.
Located within Chemical Metabolism CoreRebecca Cardone, MS
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Instrument/equipment
Invitrogen Attune NXT Cell Analyzer
Analyze cells from tubes and plates with 4 lasers (violet, blue, yellow/green, red) and 14 detectors (6 violet, 2 blue, 3 yellow, 3 red).
Located within West Campus Flow Cytometry Facility -
Instrument/equipment
Flow cytometry cell sorting
Learn more about cell sorting at Yale Flow Cytometry Facility.
Located within Yale Flow Cytometry Facility