Instrument/equipment Core/facility: Yale CryoEM Resource

Electron microscopes

Jianfeng Lin, PhD

Managing Director

About the instruments

Titan Krios (WC: Advanced Biosciences Center, room 059F; 840 West Campus Drive)
Glacios (YSM: Sterling Hall of Medicine, IE 26; 333 Cedar Street)
Glacios (SH: Yale Science Building, room C133D; 260 Whitney Avenue)
Tecnai 12 (YSM: Sterling Hall of Medicine, IE 26; 333 Cedar Street)
Talos L120C (SH: Bass Center, room 231; 266 Whitney Avenue)

300-kV Titan Krios G2 transmission electron microscope 

  • X-FEG gun
  • Constant power lenses
  • Three condenser lens system for parallel sample illumination
  • Autoloader handles and stores up to 12 EM grids
  • Volta phase plate
  • GIF Quantum LS Imaging Filter Model 1967
  • Pre-GIF CETA2 indirect detection CMOS camera
  • Pre-GIF Falcon 3EC direct detection camera
  • Post-GIF K3 summit direct detection camera
  • EPU: Automated data acquisition software for single-particle analysis
  • TEM tomography data acquisition software
  • Gatan Microscopy Suite® (GMS) 3.43
  • SerialEM (for K3) 

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200-kV YSM-Glacios

  • X-FEG gun
  • Constant power objective lens
  • Autoloader handles and stores up to 12 EM grids
  • Cryo-stage with single axis holder
  • Volta phase plate
  • CETA CMOS camera
  • K3 direct detection camera
  • EPU Software (for CETA)
  • SerialEM (for K3)

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200-kV SH-Glacios

  • X-FEG gun
  • Constant power objective lens
  • Autoloader handles and stores up to 12 EM grids
  • Cryo-stage with single axis holder
  • CETA-D CMOS camera
  • K3 summit direct detection camera
  • EPU-D Software (for MircoED)
  • SerialEM (for K3)

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120-kV YSM-Tecnai T12

  • LaB6 cathode
  • Cryo-stage with side entry holder
  • Gatan CCD camera
  • SerialEM

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120-kV SH-Talos L120C

  • LaB6 cathode
  • Constant power objective lens
  • Cryo-stage with side entry holder
  • Motorized apertures
  • Digital FluCam
  • CETA CMOS camera
  • SerialEM

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Available to Yale researchers & external researchers