CyTOF Facility

The CyTOF facility provides high-dimensional cell analysis using time-of-flight atomic mass cytometry technology. Single-cell methods detect metal conjugated antibodies on cells and tissues to provide critical advances for research in cancer, cardiovascular biology, embryonic stem cells and development, hematopoietic stem cells and progenitors, immunity and infectious disease.

• Multi-parametric single-cell data, deep profiling
• Detect up to 42 parameters simultaneously in a single sample
• Detect more markers from limited samples
• Available for suspension (Helios) and for tissue sections with imaging mass cytometry (XTi/IMC)
• Advantages over traditional flow cytometry and fluorescence microscopy: No autofluorescence, minimal to no overlap between channels, no compensation issues, simultaneous detection of many more labels in a single sample.

Instruments

• CyTOF Helios for suspension cells
• CyTOF XTi/IMC (imaging mass cytometer) for tissue sections

Resources

• Data analysis and clustering algorithms of CyTOF data is available through the Bioinformatics Service at the Yale Center for Genome Analysis. The analysis pipeline includes an initial packet of UMAPs, clustering of samples, and heatmaps for cell type annotations.
• Support from the Field Application Scientist - Standard BioTools, including monthly office hours as well as some lunch-and-learn seminars to help end users.

Biosafety policy

All infectious, human, and non-human primate cells must be fixed before they are analyzed on the CyTOF.