Standard Sequencing (1/8 Chemistry)
(on average over 1000 quality nucleotides)

Customer provides purified PCR/Plasmid template pre-mixed with one primer according to preparation guidelines

Facility:

• Adds Big Dye Terminator at 1/8 Chemistry and 5X Buffer
• Performs cycle sequencing
• Performs Dye Terminator Removal using Edge Biosystems Sephedex Columns
• Performs Genetic Analysis on 3730

Tubes-submitted in individual 1.5ml or .2ml tubes or .2ml strip tubes

96-well Plates- if plate contains empty wells customer is charged full plate price or single tube price, whichever is less

Sequencing with 1/4 Chemistry ($.50 more per sample)

Long Sequencing recovers +1000 nucleotides for fragments over 1KB

Sequencing with 1/2 Chemistry ($1.00 more per sample)

Extra Long Sequencing recovers +1000 nucleotides for fragments over 2KB (try our 1/4 chemistry first for savings)

Sequencing with 1/16 Chemistry ($.25 less per sample)

Short Length-Sequencing recovers 500-600 nucleotides

Run-Ready Sequencing

Customer provides facility with samples that have been cycle sequenced.

Facility:

• Performs Dye Terminator Removal using Edge Biosystems Sephedex Columns
  
• Performs Genetic Analysis on 3730

Customer provides facility with samples that have been cycle sequenced and purified to remove dye terminators.

Facility:

• Performs Genetic Analysis on 3730 (only)

Full Service Sequencing

Customer supplies primers and templates in separate containers with a picture of an agarose gel. We need to estimate quantity and quality.

Facility:

• Purifies PCR reactions using EXO AP
• Adds (1/8 Chemistry) Big Dye Terminator and 5X Buffer
• Performs cycle sequencing
• Performs Dye Terminator Removal using Edge Biosystems Sephedex Columns
• Performs Genetic Analysis on 3730

Fragment Analysis of Microsatellites and AFLP’s

Facility adds Size Standard (any Applied Biosystems Genescan Liz or Rox Size Standard)

Fragment Analysis of Microsatellites and AFLP’s-Ready to Run

Customer adds their own size standard